See my recent presentation at the JGI User’s meeting in Walnut Creek, CA. Really exciting and varied research presented at the meeting. Happy to be part of it!
Congratulations to Therese for being awarded a prestigious National Science Foundation Graduate Research Fellowship! Therese is currently working to establish a salt-stress regime for use in GLO-Roots, developing GWAS resources and characterizing the role of transcriptional networks in the endodermis controlling tissue differentiation under salt stress.
José has recently been elected to NAASC, the North American Arabidopsis Steering committee, along with Elizabeth Haswell from Washington University of St. Louis. Being part of NAASC provides an opportunity to organize events that promote Arabidopsis research and plant biology in general. NAASC organizes the ICAR meetings. The first duty in 2015 was to vote for St. Louis as the host city for the 2017 ICAR meeting! Meet you in St. Louis!
Heike Lindner and Therese LaRue are starting to test a newly installed automated rhizotron-handling system for GLO-Roots. The yet-to-be-named system was designed in collaboration with Modular Science, a Bay Area startup specializing in building robotic systems for biological research. The system will enable automated delivery of plants to the GLO1 imaging system as well as automated determination of weight and addition of water, luciferin, saline solution, etc. At max capacity, we will be able to image about 130 rhizotrons per day, which will enable us to conduct larger-scale experiments to understand the role of natural genetic variation in root system architecture and environmental responses. Check out our teaser video of the system.
Working with Dan Chitwood, Becky Bart and Rubén Réllan-Álvarez, we have organized a petition to gauge the support of GM technology for crop improvement by scientists and those that support a scientific approach in the development and evaluation of agricultural products. The petition is in support of the ASPB position statement on GMOs. We’ve reached over 1,400 signatories. Join the movement and leave a statement why you think it’s important to support GM technology!
Julius von Sachs is the father of modern plant physiology. Blog by Ian Street highlights importance of Sachs and describes the intellectual connectivity between the work of our lab with this scientific tradition. Sachs use of rhizotrons and the advances made through GLO-Roots are fueled by our understanding that plant biology makes sense only in an environmental context.
Open Access paper available here.
Roots are mysterious organ systems that inhabit a world hidden by a veil of soil. Methods that lift this veil often sacrifice physiological relevance in exchange for visualization capability and experimental control. Such compromises may be incompatible with our desire to understand the adaptive mechanisms plants use to thrive despite environmental changes. We have devised a new method named GLO-Roots for Growth and Luminescence Observatory for Roots. GLO-Roots comprises an integrated solution for the growth, visualization and quantification of root systems in a diverse set of plant species including the eudicots Arabidopsis and tomato as well as grasses Brachypodium distachyon and Setaria viridis (unpublished). Our method enables the visualization of plant roots in thin sheets of soil using luminescence-based reporters. We codon-optimized a wide array of luciferase reporter genes and tested which isoforms worked best to reveal the root and, in dual color reporter assays, enabled structure and gene expression to be visualized simultaneously. Using the GLO-Roots system, we were able to simulate drought conditions and showed that Arabidopsis roots exhibit enhanced gravity responses that direct growth downwards towards deep-water resources. We also show that GLO-Roots is ideal for studying root-microbe interactions through the use of bacterial luminescence reporter systems. Working with Guillame Lobet at the University of Liege, we designed GLO-RIA, an image analysis package that automatically quantifies root systems imaged using GLO-Roots. Importantly, this software is able to relate root architectural features with gene expression and soil moisture to generate a true multi-dimensional understanding of root biology. This work is of particular significance as it provides a comprehensive method to study root growth and environmental response at late stages of the lifecycle and with physiological relevance unparalleled by other published systems. The GLO-Roots method is also the subject of a patent application filed in 2013.
Heike received a post-doctoral fellowship to perform research on the response of root systems to drought stress using GLO-Roots technology. Her award comes from the Deutsche Forschungsgemeinschaft. Congratulations!
Josep has been awarded a Life Sciences Research Fellowship funded by the Simon foundation! This is a three year fellowship that will enable “Pep” to explore the moisture signaling pathway in plants using single-cell models.